| Chapter title |
Liquid Phase Multiplex High-Throughput Screening of Metagenomic Libraries Using p-Nitrophenyl-Linked Substrates for Accessory Lignocellulosic Enzymes.
|
|---|---|
| Chapter number | 13 |
| Book title |
Metagenomics
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6691-2_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6689-9, 978-1-4939-6691-2
|
| Authors |
Mariette Smart, Robert J. Huddy, Don A. Cowan, Marla Trindade |
| Editors |
Wolfgang R. Streit, Rolf Daniel |
| Abstract |
To access the genetic potential contained in large metagenomic libraries, suitable high-throughput functional screening methods are required. Here we describe a high-throughput screening approach which enables the rapid identification of metagenomic library clones expressing functional accessory lignocellulosic enzymes. The high-throughput nature of this method hinges on the multiplexing of both the E. coli metagenomic library clones and the colorimetric p-nitrophenyl linked substrates which allows for the simultaneous screening for β-glucosidases, β-xylosidases, and α-L-arabinofuranosidases. This method is readily automated and compatible with high-throughput robotic screening systems. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 2 | 13% |
| Unknown | 13 | 87% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 27% |
| Student > Master | 2 | 13% |
| Student > Ph. D. Student | 2 | 13% |
| Student > Doctoral Student | 1 | 7% |
| Student > Bachelor | 1 | 7% |
| Other | 4 | 27% |
| Unknown | 1 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 40% |
| Biochemistry, Genetics and Molecular Biology | 6 | 40% |
| Arts and Humanities | 1 | 7% |
| Business, Management and Accounting | 1 | 7% |
| Engineering | 1 | 7% |
| Other | 0 | 0% |