Metagenomics

Chapter 1 Construction of Small-Insert and Large-Insert Metagenomic Libraries.

Chapter 2 Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a cDNA as Universal Template for Marker Gene Studies.

Chapter 3 Construction and Screening of Marine Metagenomic Large Insert Libraries.

Chapter 4 Constructing and Screening a Metagenomic Library of a Cold and Alkaline Extreme Environment.

Chapter 5 DNA-, RNA-, and Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis of Active Microorganisms.

Chapter 6 Assessing Bacterial and Fungal Diversity in the Plant Endosphere.

Chapter 7 Shotgun Metagenomic Sequencing Analysis of Soft-Rot Enterobacteriaceae in Polymicrobial Communities.

Chapter 8 Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Subsequent Fermentation for Optimized Production.

Chapter 9 Degradation Network Reconstruction Guided by Metagenomic Data.

Chapter 10 Novel Tools for the Functional Expression of Metagenomic DNA.

Chapter 11 A Microtiter Plate-Based Assay to Screen for Active and Stereoselective Hydrolytic Enzymes in Enzyme Libraries.

Chapter 12 Screening for Cellulase Encoding Clones in Metagenomic Libraries.

Chapter 13 Liquid Phase Multiplex High-Throughput Screening of Metagenomic Libraries Using p-Nitrophenyl-Linked Substrates for Accessory Lignocellulosic Enzymes.

Chapter 14 Screening Glycosyltransferases for Polyphenol Modifications.

Chapter 15 Methods for the Isolation of Genes Encoding Novel PHA Metabolism Enzymes from Complex Microbial Communities.

Chapter 16 Function-Based Metagenomic Library Screening and Heterologous Expression Strategy for Genes Encoding Phosphatase Activity.

Chapter 17 Activity-Based Screening of Metagenomic Libraries for Hydrogenase Enzymes.

Chapter 18 Screening for N-AHSL-Based-Signaling Interfering Enzymes.

Chapter 19 Mining Microbial Signals for Enhanced Biodiscovery of Secondary Metabolites.