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Mendeley readers
Chapter title |
Shotgun Metagenomic Sequencing Analysis of Soft-Rot Enterobacteriaceae in Polymicrobial Communities.
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Chapter number | 7 |
Book title |
Metagenomics
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6691-2_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6689-9, 978-1-4939-6691-2
|
Authors |
James Doonan, Sandra Denman, James E. McDonald, Peter N. Golyshin |
Editors |
Wolfgang R. Streit, Rolf Daniel |
Abstract |
Shotgun metagenomic sequencing of bacterial communities in necrotic plant lesions allows insights of host-pathogen molecular interactions. Soft-rot Enterobacteriaceae are significant crop pathogens with a wide host range. Reconstructed polymicrobial community DNA from soft-rot affected crops provides details of species relative abundance and functional potential, enabling significant insights into their lifestyle. Here, we describe a workflow for DNA recovery, metagenomic shotgun sequencing and in particular, an in silico analysis of bacterial isolates from affected plant tissue. |
Mendeley readers
The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Brazil | 2 | 12% |
Unknown | 15 | 88% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 29% |
Student > Postgraduate | 3 | 18% |
Researcher | 2 | 12% |
Student > Master | 2 | 12% |
Student > Doctoral Student | 1 | 6% |
Other | 3 | 18% |
Unknown | 1 | 6% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 35% |
Agricultural and Biological Sciences | 6 | 35% |
Environmental Science | 1 | 6% |
Veterinary Science and Veterinary Medicine | 1 | 6% |
Computer Science | 1 | 6% |
Other | 0 | 0% |
Unknown | 2 | 12% |