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Attention Score in Context
Chapter title |
Activity-Based Screening of Metagenomic Libraries for Hydrogenase Enzymes.
|
---|---|
Chapter number | 17 |
Book title |
Metagenomics
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6691-2_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6689-9, 978-1-4939-6691-2
|
Authors |
Nicole Adam, Mirjam Perner |
Editors |
Wolfgang R. Streit, Rolf Daniel |
Abstract |
Here we outline how to identify hydrogenase enzymes from metagenomic libraries through an activity-based screening approach. A metagenomic fosmid library is constructed in E. coli and the fosmids are transferred into a hydrogenase deletion mutant of Shewanella oneidensis (ΔhyaB) via triparental mating. If a fosmid exhibits hydrogen uptake activity, S. oneidensis' phenotype is restored and hydrogenase activity is indicated by a color change of the medium from yellow to colorless. This new method enables screening of 48 metagenomic fosmid clones in parallel. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 31% |
Researcher | 2 | 15% |
Librarian | 1 | 8% |
Unspecified | 1 | 8% |
Student > Master | 1 | 8% |
Other | 0 | 0% |
Unknown | 4 | 31% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 31% |
Biochemistry, Genetics and Molecular Biology | 2 | 15% |
Arts and Humanities | 1 | 8% |
Unspecified | 1 | 8% |
Immunology and Microbiology | 1 | 8% |
Other | 1 | 8% |
Unknown | 3 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 February 2018.
All research outputs
#15,395,259
of 22,903,988 outputs
Outputs from Methods in molecular biology
#5,357
of 13,132 outputs
Outputs of similar age
#256,356
of 420,477 outputs
Outputs of similar age from Methods in molecular biology
#465
of 1,074 outputs
Altmetric has tracked 22,903,988 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,132 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,074 others from the same source and published within six weeks on either side of this one. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.