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Mendeley readers
Chapter title |
Development of an Assay for Measuring Proprotein-Conversion Activity on a Multiplex Magnetic Bead-Based Array Platform
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Chapter number | 31 |
Book title |
Proteomic Methods in Neuropsychiatric Research
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Published in |
Advances in experimental medicine and biology, March 2017
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DOI | 10.1007/978-3-319-52479-5_31 |
Pubmed ID | |
Book ISBNs |
978-3-31-952478-8, 978-3-31-952479-5
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Authors |
Paul C. Guest, Divya Krishnamurthy, Hassan Rahmoune |
Editors |
Paul C. Guest |
Abstract |
This chapter describes a protocol for measuring prolyl oligopeptidase (POP) activity using a biotinylated peptide substrate coupled to magnetic microspheres. The complex is incubated in the presence of a pituitary extract and activity can be detected by loss of the biotin label. The assay can be multiplexed for measuring multiple proprotein-cleaving enzymes simultaneously and can be used in analyses of neuropsychiatric diseases in which proteolytic cleavage of biologically active peptides is known to play a role. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 33% |
Student > Bachelor | 1 | 33% |
Student > Master | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 33% |
Agricultural and Biological Sciences | 1 | 33% |
Medicine and Dentistry | 1 | 33% |