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Chapter title |
A Two-Dimensional Difference Gel Electrophoresis (2D-DIGE) Protocol for Studies of Neural Precursor Cells
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Chapter number | 14 |
Book title |
Proteomic Methods in Neuropsychiatric Research
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Published in |
Advances in experimental medicine and biology, March 2017
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DOI | 10.1007/978-3-319-52479-5_14 |
Pubmed ID | |
Book ISBNs |
978-3-31-952478-8, 978-3-31-952479-5
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Authors |
Paul C. Guest |
Editors |
Paul C. Guest |
Abstract |
This chapter describes the basics of two-dimensional difference gel electrophoresis (2D-DIGE) for multiplex analysis of up to distinct proteomes. The example given describes the analysis of undifferentiated and differentiated neural precursor cells labelled with fluorescent Cy3 and Cy5 dyes in comparison to a pooled standard labelled with Cy2. After labelling, the proteomes are mixed together and electrophoresed on the same 2D gels. Scanning the gels at wavelengths specific for each dye allows direct overlay of the two different proteomes and the differences in abundance of specific protein spots can be determined through comparison to the pooled standard. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 2 | 50% |
Student > Master | 1 | 25% |
Researcher | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 25% |
Nursing and Health Professions | 1 | 25% |
Agricultural and Biological Sciences | 1 | 25% |
Medicine and Dentistry | 1 | 25% |