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Chapter title |
Sequential Immunoprecipitation of Secretory Vesicle Proteins from Biosynthetically Labelled Cells
|
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Chapter number | 11 |
Book title |
Proteomic Methods in Neuropsychiatric Research
|
Published in |
Advances in experimental medicine and biology, March 2017
|
DOI | 10.1007/978-3-319-52479-5_11 |
Pubmed ID | |
Book ISBNs |
978-3-31-952478-8, 978-3-31-952479-5
|
Authors |
Paul C. Guest |
Editors |
Paul C. Guest |
Abstract |
Pulse radiolabelling of cells with radioactive amino acids is a common method for studying the biosynthesis of proteins. The labelled proteins can then be immunoprecipitated and analysed by electrophoresis and imaging techniques. This chapter presents a protocol for the biosynthetic labelling and immunoprecipitation of pancreatic islet proteins which are known to be affected in psychiatric disorders such as schizophrenia. |