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Proteomic Methods in Neuropsychiatric Research

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Cover of 'Proteomic Methods in Neuropsychiatric Research'

Table of Contents

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    Book Overview
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    Chapter 1 Application of Proteomic Techniques for Improved Stratification and Treatment of Schizophrenia Patients
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    Chapter 2 Multiplexing Biomarker Methods, Proteomics and Considerations for Alzheimer’s Disease
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    Chapter 3 What Have Proteomic Studies Taught Us About Novel Drug Targets in Autism?
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    Chapter 4 Application of Proteomic Approaches to Accelerate Drug Development for Psychiatric Disorders
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    Chapter 5 Proteomic Biomarker Identification in Cerebrospinal Fluid for Leptomeningeal Metastases with Neurological Complications
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    Chapter 6 Connecting Brain Proteomics with Behavioural Neuroscience in Translational Animal Models of Neuropsychiatric Disorders
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    Chapter 7 LC-MSE for Qualitative and Quantitative Proteomic Studies of Psychiatric Disorders
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    Chapter 8 The Utility of Multiplex Assays for Identification of Proteomic Signatures in Psychiatry
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    Chapter 9 Blood Sampling and Preparation Procedures for Proteomic Biomarker Studies of Psychiatric Disorders
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    Chapter 10 Multiplex Immunoassay Profiling of Serum in Psychiatric Disorders
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    Chapter 11 Sequential Immunoprecipitation of Secretory Vesicle Proteins from Biosynthetically Labelled Cells
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    Chapter 12 2D Gel Electrophoresis of Insulin Secretory Granule Proteins from Biosynthetically Labelled Pancreatic Islets
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    Chapter 13 Two-Dimensional Gel Electrophoresis: A Reference Protocol
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    Chapter 14 A Two-Dimensional Difference Gel Electrophoresis (2D-DIGE) Protocol for Studies of Neural Precursor Cells
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    Chapter 15 Identifying Biomarker Candidates in the Blood Plasma or Serum Proteome
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    Chapter 16 Selective Reaction Monitoring Mass Spectrometry for Quantitation of Glycolytic Enzymes in Postmortem Brain Samples
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    Chapter 17 A Selected Reaction Monitoring Mass Spectrometry Protocol for Validation of Proteomic Biomarker Candidates in Studies of Psychiatric Disorders
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    Chapter 18 Application of iTRAQ Shotgun Proteomics for Measurement of Brain Proteins in Studies of Psychiatric Disorders
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    Chapter 19 Co-immunoprecipitation for Deciphering Protein Interactomes
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    Chapter 20 Sequential Co-immunoprecipitation and Immunoblot Approach to Determine Oligomerisation of G-Protein-Coupled Receptors
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    Chapter 21 A Clinical Study Protocol to Identify Serum Biomarkers Predictive of Response to Antipsychotics in Schizophrenia Patients
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    Chapter 22 A Protocol for Producing the Maternal Low-Protein Rat Model: A Tool for Preclinical Proteomic Studies
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    Chapter 23 Generation of the Acute Phencyclidine Rat Model for Proteomic Studies of Schizophrenia
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    Chapter 24 A Protocol for Generation of a Corticosterone Model of Psychiatric Disorders
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    Chapter 25 MK-801-Treated Oligodendrocytes as a Cellular Model to Study Schizophrenia
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    Chapter 26 Combining Patient-Reprogrammed Neural Cells and Proteomics as a Model to Study Psychiatric Disorders
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    Chapter 27 SILAC Mass Spectrometry Profiling: A Psychiatric Disorder Perspective
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    Chapter 28 Preparation of Peripheral Blood Mononuclear Cells (PBMCs) as a Model for Proteomic Studies of Psychiatric Disorders
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    Chapter 29 Proteomic Profiling of Skin Fibroblasts as a Model of Schizophrenia
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    Chapter 30 Proteomic Profiling of the Pituitary Gland in Studies of Psychiatric Disorders
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    Chapter 31 Development of an Assay for Measuring Proprotein-Conversion Activity on a Multiplex Magnetic Bead-Based Array Platform
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    Chapter 32 Phenotyping Multiple Subsets of Immune Cells In Situ in Formalin-Fixed, Paraffin-Embedded Tissue Sections
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    Chapter 33 Lab-on-a-Chip Proteomic Assays for Psychiatric Disorders
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    Chapter 34 Development of a User-Friendly App for Testing Blood Coagulation Status in Schizophrenia Patients
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    Chapter 35 Proteomic Approaches to Enable Point-of-Care Testing and Personalized Medicine for Psychiatric Disorders
Attention for Chapter 10: Multiplex Immunoassay Profiling of Serum in Psychiatric Disorders
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Chapter title
Multiplex Immunoassay Profiling of Serum in Psychiatric Disorders
Chapter number 10
Book title
Proteomic Methods in Neuropsychiatric Research
Published in
Advances in experimental medicine and biology, March 2017
DOI 10.1007/978-3-319-52479-5_10
Pubmed ID
Book ISBNs
978-3-31-952478-8, 978-3-31-952479-5
Authors

Laurie Stephen, Emanuel Schwarz, Paul C. Guest

Editors

Paul C. Guest

Abstract

Multiplex immunoassays allow for the rapid profiling of biomarker proteins in biological fluids, using less sample and labour than in single immunoassays. This chapter details the methods to develop and manufacture a 5-plex immunoassay for the Luminex® platform. Although assay development is not included here, the same methods can be used to covalently couple antibodies to the Luminex beads and to label antibodies for the screening of sandwich pairs, if needed. An example will be given for the analysis of five hormones (glucagon-like peptide 1, growth hormone, insulin, leptin and thyroid-stimulating hormone) in serum samples from schizophrenia patients and controls.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 22%
Student > Doctoral Student 2 22%
Student > Master 2 22%
Lecturer > Senior Lecturer 1 11%
Student > Ph. D. Student 1 11%
Other 0 0%
Unknown 1 11%
Readers by discipline Count As %
Medicine and Dentistry 4 44%
Neuroscience 1 11%
Nursing and Health Professions 1 11%
Unknown 3 33%