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Chapter title |
Co-immunoprecipitation for Deciphering Protein Interactomes
|
---|---|
Chapter number | 19 |
Book title |
Proteomic Methods in Neuropsychiatric Research
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Published in |
Advances in experimental medicine and biology, March 2017
|
DOI | 10.1007/978-3-319-52479-5_19 |
Pubmed ID | |
Book ISBNs |
978-3-31-952478-8, 978-3-31-952479-5
|
Authors |
Bradley J. Smith, Juliana S. Cassoli, Paul C. Guest, Daniel Martins-de-Souza |
Editors |
Paul C. Guest |
Abstract |
A single protein is often capable of binding with many partners, enabling potential effects on either protein, such as modifying its expression or activity. However, due to the complex nature of in vivo systems, it is often difficult to perform nontargeted assays with a protein of interest. Methods in discovery proteomics must be used to find potential interactors to pave the way for additional, more focused studies. This protocol describes the biological steps needed to create an interactome focused on a single protein target through co-immunoprecipitation. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 17% |
Unknown | 5 | 83% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 3 | 50% |
Researcher | 1 | 17% |
Unknown | 2 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 17% |
Medicine and Dentistry | 1 | 17% |
Unknown | 4 | 67% |