Chapter title |
A Selected Reaction Monitoring Mass Spectrometry Protocol for Validation of Proteomic Biomarker Candidates in Studies of Psychiatric Disorders
|
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Chapter number | 17 |
Book title |
Proteomic Methods in Neuropsychiatric Research
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Published in |
Advances in experimental medicine and biology, March 2017
|
DOI | 10.1007/978-3-319-52479-5_17 |
Pubmed ID | |
Book ISBNs |
978-3-31-952478-8, 978-3-31-952479-5
|
Authors |
Guilherme Reis-de-Oliveira, Sheila Garcia, Paul C. Guest, Juliana S. Cassoli, Daniel Martins-de-Souza |
Editors |
Paul C. Guest |
Abstract |
Most biomarker candidates arising from proteomic studies of psychiatric disorders have not progressed for use in clinical studies due to insufficient validation steps. Here we describe a selective reaction monitoring mass spectrometry (SRM-MS) approach that could be used as a follow-up validation tool of proteins identified in blood serum or plasma. This protocol specifically covers the stages of peptide selection and optimization. The increasing application of SRM-MS should enable fast, sensitive, and robust methods with the potential for use in clinical studies involving sampling of serum or plasma. Understanding the molecular mechanisms and identifying potential biomarkers for risk assessment, diagnosis, prognosis, and prediction of drug response goes toward the implementation of translational medicine strategies for improved treatment of patients with psychiatric disorders and other debilitating diseases. |
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Geographical breakdown
Country | Count | As % |
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Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 3 | 43% |
Student > Postgraduate | 1 | 14% |
Student > Master | 1 | 14% |
Unknown | 2 | 29% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 2 | 29% |
Nursing and Health Professions | 1 | 14% |
Business, Management and Accounting | 1 | 14% |
Medicine and Dentistry | 1 | 14% |
Unknown | 2 | 29% |