Chapter title |
SYBR® Green I-Based Fluorescence Assay to Assess Cell Viability of Malaria Parasites for Routine Use in Compound Screening
|
---|---|
Chapter number | 9 |
Book title |
Cell Viability Assays
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6960-9_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6959-3, 978-1-4939-6960-9, 978-1-4939-6959-3, 978-1-4939-6960-9
|
Authors |
Maria Leidenberger, Cornelia Voigtländer, Nina Simon, Barbara Kappes |
Editors |
Daniel F. Gilbert, Oliver Friedrich |
Abstract |
Owing to its fast and reliable assessment of parasite growth, the SYBR(®) Green I-based fluorescence assay is widely used to monitor drug susceptibility of malaria parasites. Its particular advantages are that it is a simple, one-step procedure and very cost-effective making it especially suited for high through put screening of newly developed drugs and drug combinations. Here we describe a SYBR(®) Green I-based fluorescence assay protocol to be used for routine screening of compounds and extracts in a research laboratory environment. A variation of the standard protocol is also provided allowing to address stage-specific effects of fast-acting drugs. |
X Demographics
Geographical breakdown
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 27 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 9 | 33% |
Student > Master | 3 | 11% |
Student > Postgraduate | 2 | 7% |
Student > Bachelor | 1 | 4% |
Lecturer | 1 | 4% |
Other | 2 | 7% |
Unknown | 9 | 33% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 7 | 26% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 11% |
Agricultural and Biological Sciences | 2 | 7% |
Chemistry | 2 | 7% |
Immunology and Microbiology | 1 | 4% |
Other | 1 | 4% |
Unknown | 11 | 41% |