| Chapter title |
Assaying Cellular Viability Using the Neutral Red Uptake Assay
|
|---|---|
| Chapter number | 2 |
| Book title |
Cell Viability Assays
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6960-9_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6959-3, 978-1-4939-6960-9
|
| Authors |
Gamze Ates, Tamara Vanhaecke, Vera Rogiers, Robim M. Rodrigues |
| Editors |
Daniel F. Gilbert, Oliver Friedrich |
| Abstract |
The neutral red uptake assay is a cell viability assay that allows in vitro quantification of xenobiotic-induced cytotoxicity. The assay relies on the ability of living cells to incorporate and bind neutral red, a weak cationic dye, in lysosomes. As such, cytotoxicity is expressed as a concentration-dependent reduction of the uptake of neutral red after exposure to the xenobiotic under investigation. The neutral red uptake assay is mainly used for hazard assessment in in vitro toxicology applications. This method has also been introduced in regulatory recommendations as part of 3T3-NRU-phototoxicity-assay, which was regulatory accepted in all EU member states in 2000 and in the OECD member states in 2004 as a test guideline (TG 432). The present protocol describes the neutral red uptake assay using the human hepatoma cell line HepG2, which is often employed as an alternative in vitro model for human hepatocytes. As an example, the cytotoxicity of acetaminophen and acetyl salicylic acid is assessed. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 139 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 19 | 14% |
| Researcher | 13 | 9% |
| Student > Master | 13 | 9% |
| Student > Ph. D. Student | 10 | 7% |
| Student > Doctoral Student | 6 | 4% |
| Other | 12 | 9% |
| Unknown | 66 | 47% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 20 | 14% |
| Pharmacology, Toxicology and Pharmaceutical Science | 13 | 9% |
| Chemistry | 8 | 6% |
| Agricultural and Biological Sciences | 7 | 5% |
| Medicine and Dentistry | 5 | 4% |
| Other | 10 | 7% |
| Unknown | 76 | 55% |