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Chapter title |
Metabolite Profiling of Mammalian Cell Culture Processes to Evaluate Cellular Viability
|
---|---|
Chapter number | 12 |
Book title |
Cell Viability Assays
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6960-9_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6959-3, 978-1-4939-6960-9
|
Authors |
Isobelle M. Evie, Alan J. Dickson, Mark Elvin |
Editors |
Daniel F. Gilbert, Oliver Friedrich |
Abstract |
Metabolite profiling allows for the identification of metabolites that become limiting during cell culture and/or for finding bottlenecks in metabolic pathways that limit culture growth and proliferation. Here we describe one protocol with two different sampling methodologies for GC-MS-based metabolite profiling. We also highlight an example of the types of datasets that are attainable and how such datasets can be evaluated to identify factors related to cell viability. We also demonstrate, via the same methodology, the accurate quantification of a number of metabolites of interest. |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 20 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 3 | 15% |
Student > Master | 3 | 15% |
Student > Ph. D. Student | 3 | 15% |
Lecturer | 1 | 5% |
Researcher | 1 | 5% |
Other | 1 | 5% |
Unknown | 8 | 40% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 25% |
Chemical Engineering | 1 | 5% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
Agricultural and Biological Sciences | 1 | 5% |
Psychology | 1 | 5% |
Other | 2 | 10% |
Unknown | 9 | 45% |