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Microbial Toxins

Overview of attention for book
Cover of 'Microbial Toxins'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Detection of Cholera Toxin by an Immunochromatographic Test Strip
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    Chapter 2 Electrochemical Aptamer Scaffold Biosensors for Detection of Botulism and Ricin Proteins
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    Chapter 3 A Cell-Based Fluorescent Assay to Detect the Activity of AB Toxins that Inhibit Protein Synthesis
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    Chapter 4 Molecular Methods for Identification of Clostridium tetani by Targeting Neurotoxin
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    Chapter 5 Label-Free Immuno-Sensors for the Fast Detection of Listeria in Food
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    Chapter 6 Aptamer-Based Trapping: Enrichment of Bacillus cereus Spores for Real-Time PCR Detection
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    Chapter 7 Detection of Yersinia pestis in Complex Matrices by Intact Cell Immunocapture and Targeted Mass Spectrometry
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    Chapter 8 A Method to Prepare Magnetic Nanosilicate Platelets for Effective Removal of Microcystis aeruginosa and Microcystin-LR
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    Chapter 9 An Immunochromatographic Test Strip to Detect Ochratoxin A and Zearalenone Simultaneously
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    Chapter 10 Endotoxin Removal from Escherichia coli Bacterial Lysate Using a Biphasic Liquid System
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    Chapter 11 Fourier Transform Infrared Spectroscopy as a Tool in Analysis of Proteus mirabilis Endotoxins
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    Chapter 12 Laser Interferometry Method as a Novel Tool in Endotoxins Research
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    Chapter 13 Endotoxin Entrapment on Glass via C-18 Self-Assembled Monolayers and Rapid Detection Using Drug-Nanoparticle Bioconjugate Probes
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    Chapter 14 A Bioassay for the Determination of Lipopolysaccharides and Lipoproteins
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    Chapter 15 Capillary Electrophoresis Chips for Fingerprinting Endotoxin Chemotypes and Subclasses
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    Chapter 16 Micromethods for Isolation and Structural Characterization of Lipid A, and Polysaccharide Regions of Bacterial Lipopolysaccharides
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    Chapter 17 Mass Spectrometry for Profiling LOS and Lipid A Structures from Whole-Cell Lysates: Directly from a Few Bacterial Colonies or from Liquid Broth Cultures
Attention for Chapter 10: Endotoxin Removal from Escherichia coli Bacterial Lysate Using a Biphasic Liquid System
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Chapter title
Endotoxin Removal from Escherichia coli Bacterial Lysate Using a Biphasic Liquid System
Chapter number 10
Book title
Microbial Toxins
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6958-6_10
Pubmed ID
28478561
Book ISBNs
978-1-4939-6956-2, 978-1-4939-6958-6, 978-1-4939-6956-2, 978-1-4939-6958-6
Authors

Janusz Boratyński, Bożena Szermer-Olearnik

Editors

Otto Holst

Abstract

Lipopolysaccharide (LPS, endotoxin, pyrogen) which is a component of the outer membrane of most Gram-negative bacteria is a troubling contaminant of crude bacteriophage suspension. Therefore, its removal is important for bacteriophage applications especially in preparations dedicated for use in therapy with bacterial infections treatment. The method presented here is used for extractive removal of endotoxins from bacteriophage preparations with a water immiscible solvent such as 1-octanol. During extraction most of the phage lytic activity is retained in the aqueous phase, while endotoxin accumulates in the organic solvent. The levels of endotoxin in the aqueous bacteriophage rich fraction are determine by Limulus Amebocyte Lysate or EndoLISA assay and are extremely low.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 30%
Researcher 2 20%
Student > Bachelor 1 10%
Unknown 4 40%
Readers by discipline Count As %
Agricultural and Biological Sciences 1 10%
Psychology 1 10%
Immunology and Microbiology 1 10%
Medicine and Dentistry 1 10%
Unknown 6 60%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 May 2017.
All research outputs
#20,420,242
of 22,971,207 outputs
Outputs from Methods in molecular biology
#9,928
of 13,142 outputs
Outputs of similar age
#356,025
of 421,094 outputs
Outputs of similar age from Methods in molecular biology
#842
of 1,074 outputs
Altmetric has tracked 22,971,207 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,142 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 421,094 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,074 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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