| Chapter title |
Electrochemical Aptamer Scaffold Biosensors for Detection of Botulism and Ricin Proteins
|
|---|---|
| Chapter number | 2 |
| Book title |
Microbial Toxins
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6958-6_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6956-2, 978-1-4939-6958-6
|
| Authors |
Jessica Daniel, Lisa Fetter, Susan Jett, Teisha J. Rowland, Andrew J. Bonham |
| Editors |
Otto Holst |
| Abstract |
Electrochemical DNA (E-DNA) biosensors enable the detection and quantification of a variety of molecular targets, including oligonucleotides, small molecules, heavy metals, antibodies, and proteins. Here we describe the design, electrode preparation and sensor attachment, and voltammetry conditions needed to generate and perform measurements using E-DNA biosensors against two protein targets, the biological toxins ricin and botulinum neurotoxin. This method can be applied to generate E-DNA biosensors for the detection of many other protein targets, with potential advantages over other systems including sensitive detection limits typically in the nanomolar range, real-time monitoring, and reusable biosensors. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 4% |
| Unknown | 25 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 5 | 19% |
| Student > Ph. D. Student | 4 | 15% |
| Professor | 2 | 8% |
| Researcher | 2 | 8% |
| Student > Master | 2 | 8% |
| Other | 3 | 12% |
| Unknown | 8 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 5 | 19% |
| Agricultural and Biological Sciences | 4 | 15% |
| Biochemistry, Genetics and Molecular Biology | 4 | 15% |
| Environmental Science | 2 | 8% |
| Chemical Engineering | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 9 | 35% |