| Chapter title |
Molecular Methods for Identification of Clostridium tetani by Targeting Neurotoxin
|
|---|---|
| Chapter number | 4 |
| Book title |
Microbial Toxins
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6958-6_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6956-2, 978-1-4939-6958-6
|
| Authors |
Basavraj Nagoba, Mahesh Dharne, Kushal N. Gohil |
| Editors |
Otto Holst |
| Abstract |
Tetanus is a potentially fatal muscle spasm disease. It is an important public health problem, especially in rural/tribal areas of developing countries. Tetanus toxin, a neurotoxin (tetanospasmin ), is the most important virulence factor that plays a key role in the pathogenicity of tetanus . Confirmation of virulence by confirming the production of tetanospasmin by infecting species forms the most important part in the diagnosis of tetanus . Various molecular methods have been devised for confirmation of diagnosis by targeting different genes. The most common molecular methods are tetanospasmin producing (TetX) gene-targeted methods using TetX-specific primers. Here, we describe various molecular methods targeting TetX gene such as polymerase chain reaction, pulsed-field gel electrophoresis, Southern blotting, loop-mediated isothermal amplification assay, etc. to confirm the virulence of Cl. tetani. |
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