| Chapter title |
Aptamer-Based Trapping: Enrichment of Bacillus cereus Spores for Real-Time PCR Detection
|
|---|---|
| Chapter number | 6 |
| Book title |
Microbial Toxins
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6958-6_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6956-2, 978-1-4939-6958-6
|
| Authors |
Christin Fischer, Markus Fischer, Fischer, Christin, Fischer, Markus |
| Editors |
Otto Holst |
| Abstract |
Aptamer-based trapping techniques are in general suitable to replace common antibody-based enrichment approaches. A time-consuming isolation or clean-up is often necessary during sample preparation, e.g. for the detection of spores. For the development of bioanalytical routine approaches, aptamers with a high affinity to B. cereus spores were applied for the establishment and validation of an aptamer-based trapping technique in milk with fat contents between 0.3 and 3.5%. Thereby, enrichment factors of up to sixfold were achieved. The combination of an aptamer-based enrichment by magnetic separation and the subsequent specific real-time PCR detection represents a reliable and rapid detection system. |
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|---|---|---|
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Demographic breakdown
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