| Chapter title |
Endotoxin Entrapment on Glass via C-18 Self-Assembled Monolayers and Rapid Detection Using Drug-Nanoparticle Bioconjugate Probes
|
|---|---|
| Chapter number | 13 |
| Book title |
Microbial Toxins
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6958-6_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6956-2, 978-1-4939-6958-6
|
| Authors |
Prasanta Kalita, Anshuman Dasgupta, Shalini Gupta |
| Editors |
Otto Holst |
| Abstract |
Bloodstream bacterial infections are known to illicit a systemic immune response that can lead to multiorgan failure and septic shock. The current endotoxin identification techniques in serum are expensive and elaborate requiring bulky benchtop instrumentation. We demonstrate a new route for endotoxin detection in which lipopolysaccharides (LPS) in solution are entrapped using C-18 silane-functionalized glass slides and tagged with polymyxin B sulfate (PMB) drug-conjugated gold nanoparticles. The signal from the particles is further amplified via the silver reduction approach to yield concentration-dependent colorimetric spots visible to the bare eye. The method is rapid, reliable, and cost-effective and fulfills an urgent unmet need in the healthcare industry for early septicemia diagnosis. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 3 | 43% |
| Librarian | 1 | 14% |
| Lecturer | 1 | 14% |
| Professor > Associate Professor | 1 | 14% |
| Unknown | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 4 | 57% |
| Biochemistry, Genetics and Molecular Biology | 1 | 14% |
| Engineering | 1 | 14% |
| Unknown | 1 | 14% |