| Chapter title |
Detection of Cholera Toxin by an Immunochromatographic Test Strip
|
|---|---|
| Chapter number | 1 |
| Book title |
Microbial Toxins
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6958-6_1 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6956-2, 978-1-4939-6958-6
|
| Authors |
Eiki Yamasaki, Ryuta Sakamoto, Takashi Matsumoto, Biswajit Maiti, Kayo Okumura, Fumiki Morimatsu, G. Balakrish Nair, Hisao Kurazono |
| Editors |
Otto Holst |
| Abstract |
As cholera toxin (CT) is responsible for most of the symptoms induced by Vibrio cholerae O1 or O139 infection, detection of CT is an important biomarker for diagnosis of the disease. The procedure for pathogenicity analysis of V. cholerae isolates must be carefully developed for the reason that the amount of CT produced by V. cholerae varies according to the medium used and culture conditions (i.e. temperature and aeration status) applied. Here we describe a reproducible rapid method for analysis of CT production by toxigenic V. cholerae with an immunochromatographic test strip that can detect as low as 10 ng/mL of purified recombinant CT. |
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