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Protein Tyrosine Phosphatases

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Cover of 'Protein Tyrosine Phosphatases'

Table of Contents

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    Book Overview
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    Chapter 1 The Extended Family of Protein Tyrosine Phosphatases
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    Chapter 2 Global RT-PCR and RT-qPCR Analysis of the mRNA Expression of the Human PTPome
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    Chapter 3 Expression, Purification, and Kinetic Analysis of PTP Domains
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    Chapter 4 Peptide Microarrays for Real-Time Kinetic Profiling of Tyrosine Phosphatase Activity of Recombinant Phosphatases and Phosphatases in Lysates of Cells or Tissue Samples
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    Chapter 5 Tailor-Made Protein Tyrosine Phosphatases: In Vitro Site-Directed Mutagenesis of PTEN and PTPRZ-B
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    Chapter 6 Assays to Measure PTEN Lipid Phosphatase Activity In Vitro from Purified Enzyme or Immunoprecipitates
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    Chapter 7 Assessing the Biological Activity of the Glucan Phosphatase Laforin
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    Chapter 8 Discovery and Evaluation of PRL Trimer Disruptors for Novel Anticancer Agents
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    Chapter 9 Analyzing Pseudophosphatase Function
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    Chapter 10 Crystallization of PTP Domains
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    Chapter 11 NMR Spectroscopy to Study MAP Kinase Binding to MAP Kinase Phosphatases
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    Chapter 12 Visualizing and Quantitating the Spatiotemporal Regulation of Ras/ERK Signaling by Dual-Specificity Mitogen-Activated Protein Phosphatases (MKPs)
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    Chapter 13 Protein Tyrosine Phosphatases
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    Chapter 14 Use of Dominant-Negative/Substrate Trapping PTP Mutations to Search for PTP Interactors/Substrates
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    Chapter 15 Protein Tyrosine Phosphatases
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    Chapter 16 Production of Osteoclasts for Studying Protein Tyrosine Phosphatase Signaling
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    Chapter 17 Functional Analysis of Protein Tyrosine Phosphatases in Thrombosis and Hemostasis
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    Chapter 18 Functional Analysis of Dual-Specificity Protein Phosphatases in Angiogenesis
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    Chapter 19 Studying Protein-Tyrosine Phosphatases in Zebrafish
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    Chapter 20 Protein Tyrosine Phosphatases
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    Chapter 21 Protein Tyrosine Phosphatases
Attention for Chapter 7: Assessing the Biological Activity of the Glucan Phosphatase Laforin
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Chapter title
Assessing the Biological Activity of the Glucan Phosphatase Laforin
Chapter number 7
Book title
Protein Tyrosine Phosphatases
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3746-2_7
Pubmed ID
Book ISBNs
978-1-4939-3744-8, 978-1-4939-3746-2
Authors

Carlos Romá-Mateo, Madushi Raththagala, Mathew S. Gentry, Pascual Sanz, Romá-Mateo, Carlos, Raththagala, Madushi, Gentry, Mathew S., Sanz, Pascual

Abstract

Glucan phosphatases are a recently discovered family of enzymes that dephosphorylate either starch or glycogen and are essential for proper starch metabolism in plants and glycogen metabolism in humans. Mutations in the gene encoding the only human glucan phosphatase, laforin, result in the fatal, neurodegenerative, epilepsy known as Lafora disease. Here, we describe phosphatase assays to assess both generic laforin phosphatase activity and laforin's unique glycogen phosphatase activity.

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Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 29%
Student > Bachelor 1 14%
Other 1 14%
Researcher 1 14%
Professor > Associate Professor 1 14%
Other 0 0%
Unknown 1 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 57%
Agricultural and Biological Sciences 1 14%
Unknown 2 29%