Chapter title |
Detection of the Phosphorylation of the Estrogen Receptor α as an Outcome of GPR30 Activation
|
---|---|
Chapter number | 36 |
Book title |
Estrogen Receptors
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3127-9_36 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3126-2, 978-1-4939-3127-9
|
Authors |
Sara Clark, Kevin Pollard, Jennifer Rainville, Nandini Vasudevan, Clark, Sara, Pollard, Kevin, Rainville, Jennifer, Vasudevan, Nandini |
Editors |
Kathleen M. Eyster |
Abstract |
Phosphorylation of the serine residues in estrogen receptor (ER) α is important in transcriptional activation. Hence, methods to detect such posttranslational modification events are valuable. We describe, in detail, the analysis of the phosphorylated ERα by electrophoretic separation of proteins and subsequent immuno-blotting techniques. In particular, phosphorylation of the ERα is one possible outcome of activation of the putative membrane estrogen receptor (mER), GPR30. Hence, phosphorylation represents a cross talk event between GPR30 and ERα and may be important in estrogen-regulated physiology. |
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Geographical breakdown
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United States | 1 | 11% |
Unknown | 8 | 89% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 2 | 22% |
Student > Postgraduate | 2 | 22% |
Professor | 2 | 22% |
Lecturer > Senior Lecturer | 1 | 11% |
Researcher | 1 | 11% |
Other | 1 | 11% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 2 | 22% |
Medicine and Dentistry | 2 | 22% |
Neuroscience | 1 | 11% |
Unknown | 2 | 22% |