Chapter title |
Cotranscriptional Production of Chemically Modified RNA Nanoparticles
|
---|---|
Chapter number | 6 |
Book title |
Methods in Molecular Biology
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7138-1_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7137-4, 978-1-4939-7138-1
|
Authors |
Kireeva, Maria L., Afonin, Kirill A., Shapiro, Bruce A., Kashlev, Mikhail, Maria L. Kireeva, Kirill A. Afonin, Bruce A. Shapiro, Mikhail Kashlev |
Abstract |
RNA nanoparticles consisting of multiple RNA strands of different sequences forming various three-dimensional structures emerge as promising carriers of siRNAs, RNA aptamers, and ribozymes. In vitro transcription of a mixture of dsDNA templates encoding all the subunits of the RNA nanoparticle may result in cotranscriptional self-assembly of the nanoparticle. Based on our experience with production of RNA nanorings, RNA nanocubes, and RNA three-way junctions, we propose a strategy for optimization of the cotranscriptional production of chemically modified ribonuclease-resistant RNA nanoparticles. |
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