Chapter title |
Oxime Ether Lipids as Transfection Agents: Assembly and Complexation with siRNA
|
---|---|
Chapter number | 16 |
Book title |
Methods in Molecular Biology
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7138-1_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7137-4, 978-1-4939-7138-1
|
Authors |
Puri, Anu, Zampino, Serena, Viard, Mathias, Shapiro, Bruce A., Anu Puri, Serena Zampino, Mathias Viard, Bruce A. Shapiro |
Abstract |
RNAi-based therapeutic approaches to combat cancer and other diseases are currently an area of great interest. However, practical applications of this approach rely on optimal tools to carry and deliver siRNA to the desired site. Oxime ether lipids (OELs) are a class of molecules among other various carriers being examined for siRNA delivery. OELs, relatively new candidates, belong to a class of non-glycerol based lipids and have begun to claim their place as an siRNA delivery carrier in the field of RNAi therapy. Chemical synthesis steps of OELs are considered relatively simple with the ability to modify the functionalities as desired. OEL-siRNA complexes can be assembled in the presence of serum-containing buffers (or cell culture media) and recent data from our and other groups have demonstrated that OELs are viable carriers for siRNA delivery in the cell culture systems. In this chapter, we provide the details of experimental protocols routinely used in our laboratory to examine OEL-siRNA complexes including their assembly, stability, and transfection efficiencies. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 30% |
Researcher | 3 | 30% |
Student > Doctoral Student | 1 | 10% |
Other | 1 | 10% |
Professor > Associate Professor | 1 | 10% |
Other | 0 | 0% |
Unknown | 1 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 20% |
Agricultural and Biological Sciences | 2 | 20% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 10% |
Mathematics | 1 | 10% |
Immunology and Microbiology | 1 | 10% |
Other | 2 | 20% |
Unknown | 1 | 10% |