| Chapter title |
Bisulfite Sequencing for DNA Methylation Analysis of Primary Muscle Stem Cells
|
|---|---|
| Chapter number | 1 |
| Book title |
Skeletal Muscle Development
|
| Published in |
Methods in molecular biology, August 2017
|
| DOI | 10.1007/978-1-4939-7283-8_1 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7282-1, 978-1-4939-7283-8
|
| Authors |
Miyata, Kohei, Naito, Masashi, Miyata, Tomoko, Mokuda, Sho, Asahara, Hiroshi, Kohei Miyata, Masashi Naito, Tomoko Miyata, Sho Mokuda, Hiroshi Asahara |
| Abstract |
In skeletal muscle, DNA methylation contributes to the suppression of gene expression in several biological processes and diseases. A protocol for the detection of methylated cytosine was thus established based on methylation-sensitive enzymes, immunoprecipitation, and bisulfite conversion. DNA methylation analysis, with bisulfite conversion and sequencing, enables the quantification of methylation at each single base position. Here, we describe a basic method of bisulfite sequencing that can be used to analyze local DNA methylation status to confirm genome-wide DNA methylation analysis or correlation of gene expression regulatory mechanisms. |
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| Unspecified | 2 | 8% |
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