| Chapter title |
Targeted Lipidomic Analysis of Myoblasts by GC-MS and LC-MS/MS
|
|---|---|
| Chapter number | 4 |
| Book title |
Skeletal Muscle Development
|
| Published in |
Methods in molecular biology, August 2017
|
| DOI | 10.1007/978-1-4939-7283-8_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7282-1, 978-1-4939-7283-8
|
| Authors |
Jordan Blondelle, Jean-Paul Pais de Barros, Fanny Pilot-Storck, Laurent Tiret |
| Abstract |
Lipids represent ∼10% of the cell dry mass and play essential roles in membrane composition and physical properties, energy storage, and signaling pathways. In the developing or the regenerating skeletal muscle, modifications in the content or the flipping between leaflets of membrane lipid components can modulate the fusion capacity of myoblasts, thus constituting one of the regulatory mechanisms underlying myofiber growth. Recently, few genes controlling these qualitative and quantitative modifications have started to be unraveled. The precise functional characterization of these genes requires both qualitative and quantitative evaluations of a global lipid profile. Here, we describe a lipidomic protocol using mass spectrometry, allowing assessing the content of fatty acids, glycerophospholipids, and cholesterol in the routinely used C2C12 mouse myoblast cell line, or in primary cultures of mouse myoblasts. |
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
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|---|---|---|
| Other | 2 | 15% |
| Professor | 2 | 15% |
| Student > Doctoral Student | 2 | 15% |
| Student > Ph. D. Student | 2 | 15% |
| Unspecified | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 3 | 23% |
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| Unspecified | 1 | 8% |
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| Social Sciences | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 4 | 31% |