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Mendeley readers
| Chapter title |
Application of Split-GFP Reassembly Assay to Study Myogenesis and Myofusion In Vitro
|
|---|---|
| Chapter number | 9 |
| Book title |
Skeletal Muscle Development
|
| Published in |
Methods in molecular biology, August 2017
|
| DOI | 10.1007/978-1-4939-7283-8_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7282-1, 978-1-4939-7283-8
|
| Authors |
Manami Kodaka, Xiaoyin Xu, Zeyu Yang, Junichi Maruyama, Yutaka Hata |
| Abstract |
Green fluorescent protein (GFP) is composed of 11 β-strands, and loses GFP signals, when divided into the N-terminal ten β-strands (GFP1-10) and the C-terminal last β-strand (GFP11). However, when GFP1-10 and GFP11 encounter, they reassemble into the fluorescent GFP. We expressed GFP1-10 and blasticidin resistance gene product-fused GFP11 (BSR-GFP11) in C2C12 cells. Both the cell lines do not show GFP but when they undergo myogenesis and myofusion, GFP1-10 and BSR-GFP11 form the fluorescent complex in multi-nuclear myotubes, so that GFP signals reflect myogenesis and myofusion. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 8 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 2 | 25% |
| Student > Bachelor | 1 | 13% |
| Researcher | 1 | 13% |
| Lecturer | 1 | 13% |
| Student > Master | 1 | 13% |
| Other | 0 | 0% |
| Unknown | 2 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 2 | 25% |
| Agricultural and Biological Sciences | 2 | 25% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 13% |
| Biochemistry, Genetics and Molecular Biology | 1 | 13% |
| Unknown | 2 | 25% |