Chapter title |
Profiling Nucleosome Occupancy by MNase-seq: Experimental Protocol and Computational Analysis
|
---|---|
Chapter number | 11 |
Book title |
Plant Chromatin Dynamics
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7318-7_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7317-0, 978-1-4939-7318-7
|
Authors |
Alice Pajoro, Jose M. Muiño, Gerco C. Angenent, Kerstin Kaufmann, Pajoro, Alice, Muiño, Jose M., Angenent, Gerco C., Kaufmann, Kerstin |
Abstract |
Nucleosomes are the basic repeating units of eukaryotic chromatin. They play important roles in chromatin compaction and gene regulation. Therefore, it is important to profile the in vivo locations of nucleosomes in the genome. Here we illustrate how to profile nucleosome occupancy at genome-wide scale using micrococcal nuclease (MNase) digestion combined with high throughput Illumina sequencing (MNase-seq). Nucleosome-associated DNA is relatively insensitive to digestion by micrococcal nuclease (MNase). Upon mild MNase treatment, the undigested nucleosomal DNA can be purified and sequenced allowing a precise localization of in vivo nucleosomes at a genome-wide level. |
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