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Plant Chromatin Dynamics

Overview of attention for book
Cover of 'Plant Chromatin Dynamics'

Table of Contents

  1. Altmetric Badge
    Book Overview
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    Chapter 1 Profiling Developmentally and Environmentally Controlled Chromatin Reprogramming
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    Chapter 2 Profiling DNA Methylation Using Bisulfite Sequencing (BS-Seq)
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    Chapter 3 Bisulfite Sequencing Using Small DNA Amounts
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    Chapter 4 Identification of Differentially Methylated Regions in the Genome of Arabidopsis thaliana
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    Chapter 5 A Rapid and Efficient ChIP Protocol to Profile Chromatin Binding Proteins and Epigenetic Modifications in Arabidopsis
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    Chapter 6 Sequential ChIP Protocol for Profiling Bivalent Epigenetic Modifications (ReChIP)
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    Chapter 7 A Method to Identify Nucleolus-Associated Chromatin Domains (NADs)
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    Chapter 8 Cell Type-Specific Profiling of Chromatin Modifications and Associated Proteins
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    Chapter 9 Mapping of Histone Modifications in Plants by Tandem Mass Spectrometry
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    Chapter 10 Histone H1 Purification and Post-Translational Modification Profiling by High–Resolution Mass Spectrometry
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    Chapter 11 Profiling Nucleosome Occupancy by MNase-seq: Experimental Protocol and Computational Analysis
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    Chapter 12 Identification of Open Chromatin Regions in Plant Genomes Using ATAC-Seq
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    Chapter 13 Unraveling the Complex Epigenetic Mechanisms that Regulate Gene Activity
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    Chapter 14 Technical Review: A Hitchhiker’s Guide to Chromosome Conformation Capture
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    Chapter 15 3C in Maize and Arabidopsis
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    Chapter 16 Profiling Histone Modifications in Synchronized Floral Tissues for Quantitative Resolution of Chromatin and Transcriptome Dynamics
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    Chapter 17 De Novo Identification of sRNA Loci and Non-coding RNAs by High-Throughput Sequencing
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    Chapter 18 Identification of In Planta Protein–Protein Interactions Using IP-MS
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    Chapter 19 RNA Immunoprecipitation Protocol to Identify Protein–RNA Interactions in Arabidopsis thaliana
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    Chapter 20 In Vitro Assays to Measure Histone Methyltransferase Activity Using Different Chromatin Substrates
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    Chapter 21 Identification of Parent-of-Origin-Dependent QTLs Using Bulk-Segregant Sequencing (Bulk-Seq)
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    Chapter 22 QTLepi Mapping in Arabidopsis thaliana
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    Chapter 23 A Compendium of Methods to Analyze the Spatial Organization of Plant Chromatin
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    Chapter 24 Localization of Chromatin Marks in Arabidopsis Early Embryos
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    Chapter 25 Cell-Type Specific Chromatin Analysis in Whole-Mount Plant Tissues by Immunostaining
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    Chapter 26 Measuring Dynamics of Histone Proteins by Photobleaching in Arabidopsis Roots
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    Chapter 27 Fluorescence In Situ Hybridization (FISH) and Immunolabeling on 3D Preserved Nuclei
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    Chapter 28 High-Affinity LNA–DNA Mixmer Probes for Detection of Chromosome-Specific Polymorphisms of 5S rDNA Repeats in Arabidopsis thaliana
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    Chapter 29 A Method for Testing Random Spatial Models on Nuclear Object Distributions
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    Chapter 30 Technical Review: Cytogenetic Tools for Studying Mitotic Chromosomes
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    Chapter 31 Technical Review: Microscopy and Image Processing Tools to Analyze Plant Chromatin: Practical Considerations
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    Chapter 32 Automated 3D Gene Position Analysis Using a Customized Imaris Plugin: XTFISHInsideNucleus
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    Chapter 33 Quantitative 3D Analysis of Nuclear Morphology and Heterochromatin Organization from Whole-Mount Plant Tissue Using NucleusJ
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    Chapter 34 Transmission Electron Microscopy Imaging to Analyze Chromatin Density Distribution at the Nanoscale Level
  36. Altmetric Badge
    Chapter 35 Erratum to: Bisulfite Sequencing Using Small DNA Amounts
Attention for Chapter 5: A Rapid and Efficient ChIP Protocol to Profile Chromatin Binding Proteins and Epigenetic Modifications in Arabidopsis
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (57th percentile)

Mentioned by

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5 tweeters

Citations

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5 Dimensions

Readers on

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8 Mendeley

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Chapter title
A Rapid and Efficient ChIP Protocol to Profile Chromatin Binding Proteins and Epigenetic Modifications in Arabidopsis
Chapter number 5
Book title
Plant Chromatin Dynamics
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7318-7_5
Pubmed ID
29052186
Book ISBNs
978-1-4939-7317-0, 978-1-4939-7318-7
Authors

Bénédicte Desvoyes, Zaida Vergara, Joana Sequeira-Mendes, Sofia Madeira, Crisanto Gutierrez

Abstract

Chromatin immunoprecipitation (ChIP) is a widely used and very powerful procedure to identify the proteins that are associated with the DNA to regulate developmental processes. These proteins can be transcription factors, or specific histone variants and modified histones, which are all crucial for gene regulation. In order to obtain reliable results, ChIP must be carried out under highly reproducible conditions. Here, we describe a simple and fast ChIP protocol adapted for Arabidopsis seedlings, which can serve as a basis for other species, organs or more sophisticated procedures, such as the sequential ChIP. We also provide user-oriented troubleshooting to increase the chances of successful applications.

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Twitter Demographics

The data shown below were collected from the profiles of 5 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 2 25%
Student > Ph. D. Student 2 25%
Professor 1 13%
Researcher 1 13%
Student > Postgraduate 1 13%
Other 0 0%
Unknown 1 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 38%
Agricultural and Biological Sciences 3 38%
Business, Management and Accounting 1 13%
Unknown 1 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 October 2017.
All research outputs
#7,537,383
of 14,396,570 outputs
Outputs from Methods in molecular biology
#1,906
of 8,747 outputs
Outputs of similar age
#131,786
of 318,578 outputs
Outputs of similar age from Methods in molecular biology
#1
of 1 outputs
Altmetric has tracked 14,396,570 research outputs across all sources so far. This one is in the 47th percentile – i.e., 47% of other outputs scored the same or lower than it.
So far Altmetric has tracked 8,747 research outputs from this source. They receive a mean Attention Score of 2.4. This one has done well, scoring higher than 77% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 318,578 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 57% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them

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