Chapter title |
Identification of In Planta Protein–Protein Interactions Using IP-MS
|
---|---|
Chapter number | 18 |
Book title |
Plant Chromatin Dynamics
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7318-7_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7317-0, 978-1-4939-7318-7
|
Authors |
Suraj Jamge, Gerco C. Angenent, Marian Bemer, Jamge, Suraj, Angenent, Gerco C., Bemer, Marian |
Abstract |
Gene regulation by transcription factors involves complex protein interaction networks, which include chromatin remodeling and modifying proteins as an integral part. Decoding these protein interactions is crucial for our understanding of chromatin-mediated gene regulation. Here, we describe a method for the immunoprecipitation of in planta nuclear protein complexes followed by mass spectrometry (IP-MS) to identify interactions between transcription factors and chromatin remodelers/modifiers in plants. In addition to a step-by-step bench protocol for immunoprecipitation and subsequent mass spectrometry, we provide guidelines and pointers on necessary controls and data analysis approaches. |
X Demographics
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Unknown | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 21 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 4 | 19% |
Researcher | 3 | 14% |
Student > Doctoral Student | 2 | 10% |
Unspecified | 2 | 10% |
Student > Bachelor | 2 | 10% |
Other | 2 | 10% |
Unknown | 6 | 29% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 6 | 29% |
Biochemistry, Genetics and Molecular Biology | 5 | 24% |
Unspecified | 2 | 10% |
Computer Science | 1 | 5% |
Medicine and Dentistry | 1 | 5% |
Other | 1 | 5% |
Unknown | 5 | 24% |