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| Chapter title |
RNA Immunoprecipitation Protocol to Identify Protein–RNA Interactions in Arabidopsis thaliana
|
|---|---|
| Chapter number | 19 |
| Book title |
Plant Chromatin Dynamics
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7318-7_19 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7317-0, 978-1-4939-7318-7
|
| Authors |
Benoit Mermaz, Fuquan Liu, Jie Song |
| Abstract |
The role of RNA-binding proteins in the regulation of epigenetic processes has received increasing attention in the past decades. In particular noncoding RNAs have been shown to play a role in chromatin loop formation, recruitment of chromatin modifiers and RNA-dependent DNA methylation. In plants, the identification of specific RNA-protein interactions is now rising, facilitated by the development of specific approaches for plant tissues. Here, we present a simple one-day RNA immunoprecipitation (RIP) protocol adapted for Arabidopsis, suited for the identification of RNAs that are associated with a protein-of-interest in planta. |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 20% |
| Student > Bachelor | 2 | 20% |
| Student > Master | 2 | 20% |
| Student > Ph. D. Student | 2 | 20% |
| Student > Doctoral Student | 1 | 10% |
| Other | 0 | 0% |
| Unknown | 1 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 40% |
| Agricultural and Biological Sciences | 4 | 40% |
| Nursing and Health Professions | 1 | 10% |
| Social Sciences | 1 | 10% |