| Chapter title |
Preparing cDNA Libraries from Lytic Phage-Infected Cells for Whole Transcriptome Analysis by RNA-Seq
|
|---|---|
| Chapter number | 14 |
| Book title |
Bacteriophages
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7343-9_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7341-5, 978-1-4939-7343-9
|
| Authors |
Bob Blasdel, Pieter-Jan Ceyssens, Rob Lavigne, Blasdel, Bob, Ceyssens, Pieter-Jan, Lavigne, Rob |
| Abstract |
Whole genome wide analysis of transcription using RNA-Seq methods is a powerful way to elucidate differential expression of gene features in bacteria across different conditions as well as for discovering previously exotic RNA species. Indeed, RNA sequencing has revolutionized the study of bacterial transcription with the diversity and quantity of small noncoding RNA elements that have been found and its ability to clearly define operons, promoters , and terminators . We discuss our experience with applying RNA sequencing technology to analyzing the lytic cycle, including extraction, processing, and a guide to the customized statistical analysis necessary for analyzing differential host and phage transcription. |
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|---|---|---|
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| France | 2 | 18% |
| Belgium | 1 | 9% |
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| Unknown | 2 | 18% |
Demographic breakdown
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|---|---|---|
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| Scientists | 2 | 18% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 35 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 29% |
| Student > Master | 6 | 17% |
| Researcher | 5 | 14% |
| Professor | 1 | 3% |
| Student > Bachelor | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 11 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Immunology and Microbiology | 4 | 11% |
| Environmental Science | 2 | 6% |
| Veterinary Science and Veterinary Medicine | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 11 | 31% |