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Mendeley readers
| Chapter title |
Reconstitution and Transport Analysis of Eukaryotic Transporters in the Post-Genomic Era
|
|---|---|
| Chapter number | 19 |
| Book title |
Bacterial Multidrug Exporters
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7454-2_19 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7452-8, 978-1-4939-7454-2
|
| Authors |
Hiroshi Omote, Yoshinori Moriyama |
| Abstract |
Measuring transport activity through reconstituted proteoliposomes is a key technique to resolve numerous problems found in the traditional methods. The system includes overexpression, purification, and reconstitution of transporters. Mixing of purified transporter with lipid and dilution below the critical micelle concentration result in rapid generation of proteoliposomes. Incubation of proteoliposomes in the presence of a driving force initiates substrate uptake. After starting the reaction, samples are passed through a gel filtration column to separate proteoliposomes from the reaction mixture. Here, we describe step-by-step procedures for such reconstitution assays. |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Lecturer | 1 | 11% |
| Student > Bachelor | 1 | 11% |
| Student > Ph. D. Student | 1 | 11% |
| Student > Master | 1 | 11% |
| Researcher | 1 | 11% |
| Other | 1 | 11% |
| Unknown | 3 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 44% |
| Immunology and Microbiology | 1 | 11% |
| Medicine and Dentistry | 1 | 11% |
| Unknown | 3 | 33% |