↓ Skip to main content
What is this page? Embed badge

The Retinoblastoma Protein

Overview of attention for book
Cover of 'The Retinoblastoma Protein'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Characterization of RB1 Deletions in Interphase and Metaphase by Molecular Cytogenetics Exemplified in Chronic Lymphatic Leukemia
  3. Altmetric Badge
    Chapter 2 Detection of RB1 Gene Copy Number Variations Using a Multiplex Ligation-Dependent Probe Amplification Method
  4. Altmetric Badge
    Chapter 3 A Fluorescent Quantitative Multiplex PCR Method to Detect Copy Number Changes in the RB1 Gene
  5. Altmetric Badge
    Chapter 4 Using Methylation-Specific PCR to Study RB1 Promoter Hypermethylation
  6. Altmetric Badge
    Chapter 5 Detection of Aberrant DNA Methylation Patterns in the RB1 Gene
  7. Altmetric Badge
    Chapter 6 Detection of Retinoblastoma Protein Phosphorylation by Immunoblot Analysis
  8. Altmetric Badge
    Chapter 7 Immunohistochemical Detection of the Retinoblastoma Protein
  9. Altmetric Badge
    Chapter 8 Immunohistochemical Detection of Retinoblastoma Protein Phosphorylation in Human Tumor Samples
  10. Altmetric Badge
    Chapter 9 Detection of CCND1 Locus Amplification by Fluorescence In Situ Hybridization
  11. Altmetric Badge
    Chapter 10 Detection of CCND1 Gene Copy Number Variations Using Multiplex Ligation-Dependent Probe Amplification and Fluorescence In Situ Hybridization Methods
  12. Altmetric Badge
    Chapter 11 Detection of p16 Promoter Hypermethylation by Methylation-Specific PCR
  13. Altmetric Badge
    Chapter 12 Immunohistochemical Detection of p16 in Clinical Samples
  14. Altmetric Badge
    Chapter 13 Detection of E2F-DNA Complexes Using Chromatin Immunoprecipitation Assays
  15. Altmetric Badge
    Chapter 14 Detection of E2F-Induced Transcriptional Activity Using a Dual Luciferase Reporter Assay
  16. Altmetric Badge
    Chapter 15 Detection of HPV E6/E7 mRNA in Clinical Samples Using RNA In Situ Hybridization
  17. Altmetric Badge
    Chapter 16 CRISPR/Cas9-Mediated Knockout of Rb1 in Xenopus tropicalis
Attention for Chapter 16: CRISPR/Cas9-Mediated Knockout of Rb1 in Xenopus tropicalis
Altmetric Badge

About this Attention Score

  • Average Attention Score compared to outputs of the same age and source

Mentioned by

twitter
2 X users

Readers on

mendeley
18 Mendeley
Summary X
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
CRISPR/Cas9-Mediated Knockout of Rb1 in Xenopus tropicalis
Chapter number 16
Book title
The Retinoblastoma Protein
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7565-5_16
Pubmed ID
29468553
Book ISBNs
978-1-4939-7564-8, 978-1-4939-7565-5
Authors

Thomas Naert, Kris Vleminckx

Abstract

At this time, no molecular targeted therapies exist for treatment of retinoblastoma. This can be, in part, attributed to the lack of animal models that allow for both rapid identification of novel therapeutic targets and hypothesis driven drug testing. Within this scope, we have recently reported the first genuine genetic nonmammalian retinoblastoma cancer model within the aquatic model organism Xenopus tropicalis (Naert et al., Sci Rep 6: 35263, 2016). Here we describe the methods to generate rb1 mosaic mutant Xenopus tropicalis by employing the CRISPR/Cas9 technology. In depth, we discuss short guide RNA (sgRNA) design parameters, generation, quality control, quantification, and delivery followed by several methods for assessing genome editing efficiencies. As such the reader should be capable, by minor changes to the methods described here, to (co-) target rb1 or any one or multiple gene(s) within the Xenopus tropicalis genome by multiplex CRISPR/Cas9 methodology.

View on publisher site
X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 18 100%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 2 11%
Lecturer 2 11%
Student > Bachelor 2 11%
Student > Ph. D. Student 2 11%
Professor 2 11%
Other 3 17%
Unknown 5 28%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 22%
Neuroscience 4 22%
Agricultural and Biological Sciences 3 17%
Chemical Engineering 1 6%
Nursing and Health Professions 1 6%
Other 2 11%
Unknown 3 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 February 2018.
All research outputs
#17,932,482
of 23,025,074 outputs
Outputs from Methods in molecular biology
#7,289
of 13,170 outputs
Outputs of similar age
#310,401
of 442,364 outputs
Outputs of similar age from Methods in molecular biology
#869
of 1,499 outputs
Altmetric has tracked 23,025,074 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,170 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,364 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,499 others from the same source and published within six weeks on either side of this one. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.

This page is provided by Altmetric.