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Mendeley readers
| Chapter title |
Immunohistochemical Detection of Retinoblastoma Protein Phosphorylation in Human Tumor Samples
|
|---|---|
| Chapter number | 8 |
| Book title |
The Retinoblastoma Protein
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7565-5_8 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7564-8, 978-1-4939-7565-5
|
| Authors |
Jaileene Pérez-Morales, Angel Núñez-Marrero, Pedro G. Santiago-Cardona |
| Abstract |
The retinoblastoma protein (pRb) is an important tumor suppressor and cell cycle repressor. pRb is a phosphoprotein whose function is regulated primarily at the level of phosphorylation, and therefore, detecting pRb's phosphorylation status in human tissue samples can be clinically informative. Unfortunately, detection of phosphorylated pRb residues can be technically challenging, as these residues can often be weak antigens. In this chapter, we describe an enhanced sensitivity immunohistochemistry protocol for the staining of phosphorylated serine 249 in pRb, in human lung tumor samples. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Unspecified | 1 | 17% |
| Student > Ph. D. Student | 1 | 17% |
| Lecturer | 1 | 17% |
| Unknown | 3 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Unspecified | 1 | 17% |
| Biochemistry, Genetics and Molecular Biology | 1 | 17% |
| Unknown | 4 | 67% |