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Programmed Cell Death

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Cover of 'Programmed Cell Death'

Table of Contents

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    Book Overview
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    Chapter 1 Detection of Apoptotic Versus Autophagic Cell Death by Flow Cytometry
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    Chapter 2 In Vivo Apoptosis Imaging Using Site-Specifically 68 Ga-Labeled Annexin V
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    Chapter 3 Detection of Active Caspases During Apoptosis Using Fluorescent Activity-Based Probes
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    Chapter 4 Detection of Initiator Caspase Induced Proximity in Single Cells by Caspase Bimolecular Fluorescence Complementation
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    Chapter 5 In Vitro Use of Peptide Based Substrates and Inhibitors of Apoptotic Caspases
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    Chapter 6 Programmed Cell Death
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    Chapter 7 Analysis of Cell Death Induction in Intestinal Organoids In Vitro
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    Chapter 8 In Vitro Differentiation of Mouse Granulocytes
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    Chapter 9 Programmed Cell Death
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    Chapter 10 Isolation of Cardiomyocytes and Cardiofibroblasts for Ex Vivo Analysis
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    Chapter 11 Programmed Cell Death
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    Chapter 12 Programmed Cell Death
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    Chapter 13 Modeling Metazoan Apoptotic Pathways in Yeast
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    Chapter 14 Characterizing Bcl-2 Family Protein Conformation and Oligomerization Using Cross-Linking and Antibody Gel-Shift in Conjunction with Native PAGE
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    Chapter 15 Using Förster-Resonance Energy Transfer to Measure Protein Interactions Between Bcl-2 Family Proteins on Mitochondrial Membranes
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    Chapter 16 Preparing Samples for Crystallization of Bcl-2 Family Complexes
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    Chapter 17 Programmed Cell Death
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    Chapter 18 Programmed Cell Death
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    Chapter 19 Programmed Cell Death
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    Chapter 20 Proteomic Profiling of Cell Death: Stable Isotope Labeling and Mass Spectrometry Analysis
Attention for Chapter 16: Preparing Samples for Crystallization of Bcl-2 Family Complexes
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Chapter title
Preparing Samples for Crystallization of Bcl-2 Family Complexes
Chapter number 16
Book title
Programmed Cell Death
Published in
Methods in molecular biology, April 2016
DOI 10.1007/978-1-4939-3581-9_16
Pubmed ID
Book ISBNs
978-1-4939-3579-6, 978-1-4939-3581-9
Authors

Kvansakul, Marc, Czabotar, Peter E., Marc Kvansakul, Peter E. Czabotar

Abstract

High-resolution protein structures determined by X-ray crystallography or NMR have proven invaluable for deciphering the molecular mechanisms underlying the function of a vast range of proteins. Here, we describe methods to generate complexes of proteins belonging to the Bcl-2 family of proteins with either biological ligands or small molecule antagonists.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Professor > Associate Professor 1 25%
Student > Bachelor 1 25%
Researcher 1 25%
Student > Postgraduate 1 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 75%
Chemistry 1 25%