You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
| Chapter title |
Detection of Active Caspases During Apoptosis Using Fluorescent Activity-Based Probes
|
|---|---|
| Chapter number | 3 |
| Book title |
Programmed Cell Death
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3581-9_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3579-6, 978-1-4939-3581-9
|
| Authors |
Laura E. Edgington-Mitchell, Matthew Bogyo, Edgington-Mitchell, Laura E., Bogyo, Matthew |
| Abstract |
Activity-based probes (ABPs) are reactive small molecules that covalently bind to active enzymes. When tagged with a fluorophore, ABPs serve as powerful tools to investigate enzymatic activity across a wide variety of applications. In this chapter, we provide detailed methods for using fluorescent ABPs to detect the activity of caspases during the onset of apoptosis in vitro. We describe how these probes can be used to biochemically profile caspase activity in vitro using fluorescent SDS-PAGE as well as their application to imaging protease activity in live animals and tissues. |
Mendeley readers
The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 16 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 6 | 38% |
| Student > Ph. D. Student | 3 | 19% |
| Researcher | 2 | 13% |
| Student > Master | 1 | 6% |
| Professor > Associate Professor | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 3 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 31% |
| Agricultural and Biological Sciences | 2 | 13% |
| Chemistry | 2 | 13% |
| Immunology and Microbiology | 1 | 6% |
| Philosophy | 1 | 6% |
| Other | 2 | 13% |
| Unknown | 3 | 19% |