| Chapter title |
In Vitro Differentiation of Mouse Granulocytes
|
|---|---|
| Chapter number | 8 |
| Book title |
Programmed Cell Death
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3581-9_8 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3579-6, 978-1-4939-3581-9
|
| Authors |
Ramona Reinhart, Simone Wicki, Thomas Kaufmann, Reinhart, Ramona, Wicki, Simone, Kaufmann, Thomas |
| Abstract |
Granulocytes are central players of the immune system and, once activated, a tightly controlled balance between effector functions and cell removal by apoptosis guarantees maximal host benefit with least possible collateral damage to healthy tissue.Granulocytes are terminally differentiated cells that cannot be maintained in culture for prolonged times. Isolating primary granulocytes is inefficient and challenging when working with mice, and especially so for the lowly abundant eosinophil and basophil subtypes. Here we describe an in vitro protocol to massively expand mouse derived myeloid progenitors and to differentiate them "on demand" and in large numbers into mature neutrophils or basophils. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 55 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 20% |
| Student > Master | 8 | 15% |
| Student > Bachelor | 6 | 11% |
| Student > Doctoral Student | 5 | 9% |
| Researcher | 5 | 9% |
| Other | 8 | 15% |
| Unknown | 12 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 12 | 22% |
| Agricultural and Biological Sciences | 10 | 18% |
| Immunology and Microbiology | 8 | 15% |
| Medicine and Dentistry | 7 | 13% |
| Engineering | 3 | 5% |
| Other | 4 | 7% |
| Unknown | 11 | 20% |