Chapter title |
Isolation of Cardiomyocytes and Cardiofibroblasts for Ex Vivo Analysis
|
---|---|
Chapter number | 10 |
Book title |
Programmed Cell Death
|
Published in |
Methods in molecular biology, April 2016
|
DOI | 10.1007/978-1-4939-3581-9_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3579-6, 978-1-4939-3581-9
|
Authors |
Mbogo, George Williams, Nedeva, Christina, Puthalakath, Hamsa, George Williams Mbogo, Christina Nedeva, Hamsa Puthalakath |
Abstract |
Heart failure (HF) is a common clinical endpoint to several underlying causes including aging, hypertension, stress, and cardiomyopathy. It is characterized by a significant decline in the cardiac output. Cardiomyocytes are terminally differentiated cells and therefore, apoptotic death due to beta adrenergic (β-AR) signaling contributes to high attrition rate of these cells. Past treatments of HF offer some survival benefit to patients (e.g., the beta blockers), but at the expense of blocking the compensatory beta-adrenergic signaling in surviving cells. One prerequisite for developing new therapeutics is to be able to grow cardiomyocytes ex vivo, and test their apoptotic response to drugs. Here we describe methods for isolation and culturing of neonatal and adult calcium tolerant cardiomyocytes. Similarly, cardiofibroblasts can also be isolated using the same protocol and subsequently, immortalized with SV40 T-Antigen for ex vivo studies. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 2 | 25% |
Student > Bachelor | 2 | 25% |
Researcher | 2 | 25% |
Student > Master | 2 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 75% |
Nursing and Health Professions | 1 | 13% |
Engineering | 1 | 13% |