↓ Skip to main content

Single Cell Protein Analysis

Overview of attention for book
Cover of 'Single Cell Protein Analysis'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Single-Cell Western Blotting
  3. Altmetric Badge
    Chapter 2 A Microfluidic Device for Immunoassay-Based Protein Analysis of Single E. coli Bacteria
  4. Altmetric Badge
    Chapter 3 Enzyme-Linked ImmunoSpot (ELISpot) for Single-Cell Analysis
  5. Altmetric Badge
    Chapter 4 Single Cell Protein Analysis
  6. Altmetric Badge
    Chapter 5 Single Cell Protein Analysis
  7. Altmetric Badge
    Chapter 6 Microfluidic Flow Cytometry for Single-Cell Protein Analysis
  8. Altmetric Badge
    Chapter 7 Microfluidic Image Cytometry for Single-Cell Phenotyping of Human Pluripotent Stem Cells
  9. Altmetric Badge
    Chapter 8 Characterizing Phenotypes and Signaling Networks of Single Human Cells by Mass Cytometry.
  10. Altmetric Badge
    Chapter 9 Multiplexed Peptide-MHC Tetramer Staining with Mass Cytometry.
  11. Altmetric Badge
    Chapter 10 Imaging and Mapping of Tissue Constituents at the Single-Cell Level Using MALDI MSI and Quantitative Laser Scanning Cytometry
  12. Altmetric Badge
    Chapter 11 SPLIFF: A Single-Cell Method to Map Protein-Protein Interactions in Time and Space.
  13. Altmetric Badge
    Chapter 12 Microfluidic Proximity Ligation Assay for Profiling Signaling Networks with Single-Cell Resolution
  14. Altmetric Badge
    Chapter 13 Dynamics and Interactions of Individual Proteins in the Membrane of Single, Living Cells
  15. Altmetric Badge
    Chapter 14 Microfluidics-Enabled Enzyme Activity Measurement in Single Cells
  16. Altmetric Badge
    Chapter 15 Microfluidic Chemical Cytometry for Enzyme Assays of Single Cells
  17. Altmetric Badge
    Chapter 16 Quantitative Detection of Nucleocytoplasmic Transport of Native Proteins in Single Cells
Attention for Chapter 1: Single-Cell Western Blotting
Altmetric Badge

Citations

dimensions_citation
2 Dimensions

Readers on

mendeley
11 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Single-Cell Western Blotting
Chapter number 1
Book title
Single Cell Protein Analysis
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2987-0_1
Pubmed ID
Book ISBNs
978-1-4939-2986-3, 978-1-4939-2987-0
Authors

Elly Sinkala, Amy E. Herr

Abstract

Little headway has been made in single cell protein analysis, aside from tools that rely solely on antibody-probe based detection (i.e., flow cytometry, immunocytochemistry), which are limited by low specificity and multiplexing capabilities. To address these protein analysis gaps, we have introduced a single-cell western blot (scWestern). The protein assay is capable of highly specific analysis by coupling antibody-based detection with a polyacrylamide gel electrophoresis (PAGE) protein separation. Cells are settled via gravity into polyacrylamide (PA) microwells, chemically lysed in the wells, and then subjected to PAGE through the walls of the microwells and into the surrounding PA gel. Over a thousand single-cell separations are performed simultaneously, and multiple protein targets of interest are investigated. After PAGE separation, photo-immobilization of all proteins to the gel allows for antibody probing and lends to the archival quality of the scWestern assay where new proteins targets can be investigated months after the initial separations are performed.

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Sweden 1 9%
Unknown 10 91%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 18%
Researcher 2 18%
Student > Master 2 18%
Other 1 9%
Student > Bachelor 1 9%
Other 1 9%
Unknown 2 18%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 45%
Engineering 3 27%
Medicine and Dentistry 1 9%
Unknown 2 18%