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Detection of Blotted Proteins

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Cover of 'Detection of Blotted Proteins'

Table of Contents

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    Book Overview
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    Chapter 1 Western Blotting: Origin and Ascent of the Species.
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    Chapter 2 Methods to Concentrate Proteins for Protein Isolation, Proteomic, and Peptidomic Evaluation.
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    Chapter 3 Measuring Protein Concentration on Nitrocellulose and After the Electrophoretic Transfer of Protein to Nitrocellulose
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    Chapter 4 Detection of Blotted Proteins: Not All Blockers Are Created Equal
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    Chapter 5 Protein Stains to Detect Antigen on Membranes
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    Chapter 6 Fluorescent Labeling of Proteins and Its Application to SDS-PAGE and Western Blotting
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    Chapter 7 Rapid, Antibody-Free Detection of Recombinant Proteins on Blots Using Enzyme Fragment Complementation
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    Chapter 8 Use of Nonradioactive Detection Method for North- and South-Western Blot
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    Chapter 9 Immunoblotting Using Radiolabeled Reagents for Detection
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    Chapter 10 Immunoblotting of Antigens: Whole, Strip, and New-Line Nitrocellulose Membrane Immunoblotting Using the Chemiluminescence Technique
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    Chapter 11 Detection of Protein Carbonyls by Means of Biotin Hydrazide–Streptavidin Affinity Methods
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    Chapter 12 Direct Immunodetection of Antigens Within the Precast Polyacrylamide Gel
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    Chapter 13 Quantitative Analysis of Signal Transduction with In-Cell Western Immunofluorescence Assays
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    Chapter 14 Ultrasensitive Protein Detection on Dot Blots and Western Blots with Semiconducting Polymer Dots
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    Chapter 15 Co-detection of Target and Total Protein by CyDye Labeling and Fluorescent ECL Plex Immunoblotting in a Standard Proteomics Workflow.
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    Chapter 16 Using Biotinylated Proteins to Demonstrate Immunodetection of Antigens via Western Blotting, Dot Blots, and Immunohistochemistry
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    Chapter 17 Calcium Binding by Ro 60 Multiple Antigenic Peptides on PVDF Membrane
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    Chapter 18 Sequential Use of Immunoblots for Characterization of Autoantibody Specificities
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    Chapter 19 Nanogold Immunodetection Detection Systems for the Identification of Autoantigens by Western Blotting
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    Chapter 20 Application of Intermittent Microwave Irradiation to Western Blot Analysis
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    Chapter 21 Visualization of Unstained Protein Bands on PVDF
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    Chapter 22 Multiplexed Fluorescent Immunodetection Using Low Autofluorescence Immobilon ® -FL Membrane
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    Chapter 23 Cold Microwave-Enabled Protein Detection and Quantification
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    Chapter 24 TLC-Blot (Far-Eastern Blot) and Its Application to Functional Lipidomics
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    Chapter 25 Analysis of Electroblotted Proteins by Mass Spectrometry.
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    Chapter 26 On-Membrane Renaturation of Recombinant Ro60 Autoantigen by Calcium Ions
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    Chapter 27 Phosphoprotein Detection on Protein Electroblot Using a Phosphate-Specific Fluorophore
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    Chapter 28 Purification of Tryptic Digests on Polyvinylidene Difluoride Membrane
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    Chapter 29 Detection of Blotted Proteins on Nitrocellulose/PVDF Membranes by Alta
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    Chapter 30 Nonstripping “Rainbow” and Multiple Antigen Detection (MAD) Western Blotting
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    Chapter 31 Supported Molecular Matrix Electrophoresis
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    Chapter 32 Parafilm-M(®), An Available Cost-Effective Alternative for Immuno-blot Pouches.
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    Chapter 33 Succinylation-Alcian Blue Staining of Mucins on Polyvinylidene Difluoride Membranes
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    Chapter 34 Comparison of Chemiluminescence vs. Infrared Techniques for Detection of Fetuin-A in Saliva
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    Chapter 35 A Novel Methodology for Stripping and Reprobing of Western Blots Originally Developed with Colorimetric Substrate TMB
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    Chapter 36 Other Notable Methods of Membrane Protein Detection: A Brief Review
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    Chapter 37 Nitrocellulose Membrane: The New Canvas
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    Chapter 38 Invisible Ink Marking in ECL Membrane Assays.
Attention for Chapter 37: Nitrocellulose Membrane: The New Canvas
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Chapter title
Nitrocellulose Membrane: The New Canvas
Chapter number 37
Book title
Detection of Blotted Proteins
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2718-0_37
Pubmed ID
Book ISBNs
978-1-4939-2717-3, 978-1-4939-2718-0
Authors

Jasmin R. Kurien, Bianca A. Kurien

Abstract

The use of nitrocellulose membranes for invisible ink-messaging or marking for orientation purposes has been carried out with diluted alkaline phosphatase, followed by development with nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate and chemiluminescence detection systems. Here, we show that nitrocellulose membrane can serve as a canvas for art using alkaline phosphatase and NBT/BCIP detection method.

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 1 33%
Researcher 1 33%
Unknown 1 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 33%
Chemistry 1 33%
Unknown 1 33%