Application of Intermittent Microwave Irradiation to Western Blot Analysis
Detection of Blotted Proteins
Methods in molecular biology, January 2015
Yu-Ting Liu, Shinya Toyokuni
We established a shortened protocol for Western blot analysis using intermittent microwave irradiation. With this method, the procedure is completed within 1 h after applying the primary antibody, and thus greatly saves time. This procedure appears to be applicable to any antibody based on our experience of several years.
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