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Mendeley readers
Chapter title |
Sequential Use of Immunoblots for Characterization of Autoantibody Specificities
|
---|---|
Chapter number | 18 |
Book title |
Detection of Blotted Proteins
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Published in |
Methods in molecular biology, January 2015
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DOI | 10.1007/978-1-4939-2718-0_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2717-3, 978-1-4939-2718-0
|
Authors |
Holger Bartsch, Michael P. Bachmann, Bartsch, Holger, Bachmann, Michael P. |
Abstract |
Sera of patients with systemic autoimmune diseases frequently contain autoantibodies to nuclear autoantigens. Immunoblotting of recombinant and native autoantigens is a commonly used technique for the identification and characterization of autoantibody specificities. Here we describe an easy procedure which facilitates the comparison of antibody specificities by reusing the same immunoblot for at least three times in order to detect an abundantly expressed autoantigen in total cellular extracts. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 25% |
Professor > Associate Professor | 1 | 25% |
Student > Bachelor | 1 | 25% |
Researcher | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 3 | 75% |
Medicine and Dentistry | 1 | 25% |