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Mendeley readers
| Chapter title |
Analyses of Plant UDP-Dependent Glycosyltransferases to Identify Their Volatile Substrates Using Recombinant Proteins
|
|---|---|
| Chapter number | 16 |
| Book title |
Plant Signal Transduction
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3115-6_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3114-9, 978-1-4939-3115-6
|
| Authors |
Yusuke Kamiyoshihara, Denise M. Tieman, Harry J. Klee |
| Abstract |
Glycosylation is one of major modifications for plant secondary metabolites. In the case of volatile compounds, glycosylation makes them nonvolatile and odorless. Identification of UDP-dependent glycosyltransferases responsible for volatile glycosylation is essential to understand the regulatory mechanism of volatile release from plant tissues. Here, we describe an efficient protocol to find possible combinations of volatiles/glycosyltransferases using tomato (Solanum lycopersicum) enzymes expressed in Escherichia coli. The presented method requires a basic gas chromatography system and conventional laboratory tools. |
Mendeley readers
The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 3 | 27% |
| Student > Bachelor | 2 | 18% |
| Student > Ph. D. Student | 2 | 18% |
| Unspecified | 1 | 9% |
| Student > Master | 1 | 9% |
| Other | 2 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 55% |
| Chemistry | 3 | 27% |
| Unspecified | 1 | 9% |
| Unknown | 1 | 9% |