Chapter title |
Preparation of Epidermal Peels and Guard Cell Protoplasts for Cellular, Electrophysiological, and -Omics Assays of Guard Cell Function
|
---|---|
Chapter number | 9 |
Book title |
Plant Signal Transduction
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3115-6_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3114-9, 978-1-4939-3115-6
|
Authors |
Mengmeng Zhu, Byeong Wook Jeon, Sisi Geng, Yunqing Yu, Kelly Balmant, Sixue Chen, Sarah M. Assmann, Zhu, Mengmeng, Jeon, Byeong Wook, Geng, Sisi, Yu, Yunqing, Balmant, Kelly, Chen, Sixue, Assmann, Sarah M. |
Abstract |
Bioassays are commonly used to study stomatal phenotypes. There are multiple options in the choice of plant materials and species used for observation of stomatal and guard cell responses in vivo. Here, detailed procedures for bioassays of stomatal responses to abscisic acid (ABA) in Arabidopsis thaliana are described, including ABA promotion of stomatal closure, ABA inhibition of stomatal opening, and ABA promotion of reaction oxygen species (ROS) production in guard cells. We also include an example of a stomatal bioassay for the guard cell CO2 response using guard cell-enriched epidermal peels from Brassica napus. Highly pure preparations of guard cell protoplasts can be produced, which are also suitable for studies on guard cell signaling, as well as for studies on guard cell ion transport. Small-scale and large-scale guard cell protoplast preparations are commonly used for electrophysiological and -omics studies, respectively. We provide a procedure for small-scale guard cell protoplasting from A. thaliana. Additionally, a general protocol for large-scale preparation of guard cell protoplasts, with specifications for three different species, A. thaliana, B. napus, and Vicia faba is also provided. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 33 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 8 | 24% |
Researcher | 7 | 21% |
Student > Bachelor | 4 | 12% |
Student > Doctoral Student | 3 | 9% |
Student > Master | 2 | 6% |
Other | 4 | 12% |
Unknown | 5 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 16 | 48% |
Biochemistry, Genetics and Molecular Biology | 7 | 21% |
Unspecified | 1 | 3% |
Psychology | 1 | 3% |
Earth and Planetary Sciences | 1 | 3% |
Other | 1 | 3% |
Unknown | 6 | 18% |