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Chapter title |
Application of Variable Angle Total Internal Reflection Fluorescence Microscopy to Investigate Protein Dynamics in Intact Plant Cells
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Chapter number | 10 |
Book title |
Plant Signal Transduction
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-3115-6_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3114-9, 978-1-4939-3115-6
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Authors |
Yinglang Wan, Yiqun Xue, Ruili Li, Jinxing Lin, Wan, Yinglang, Xue, Yiqun, Li, Ruili, Lin, Jinxing |
Abstract |
Variable angle total internal reflection fluorescence microscopy (VA-TIRFM) is an optical method to observe the molecular events occurring in an extremely thin region near the plasma membrane. Recently, the VA-TIRFM technique has been widely used to study fluorescently labeled target molecules in living animal and plant cells. Here, we describe the optical principle of the VA-TIRFM technique and provide a detailed experimental procedure for the study of living plant cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 33% |
Student > Doctoral Student | 1 | 33% |
Unknown | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Physics and Astronomy | 1 | 33% |
Neuroscience | 1 | 33% |
Unknown | 1 | 33% |