Chapter title |
The Biochemical Properties of Antibodies and Their Fragments
|
---|---|
Chapter number | 1 |
Book title |
ELISA
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2742-5_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2741-8, 978-1-4939-2742-5
|
Authors |
Robert M. Hnasko, Hnasko, Robert M. |
Abstract |
Immunoglobulins (Ig) or antibodies are powerful molecular recognition tools that can be used to identify minute quantities of a given target analyte. Their antigen-binding properties define both the sensitivity and selectivity of an immunoassay. Understanding the biochemical properties of this class of protein will provide users with the knowledge necessary to select the appropriate antibody composition to maximize immunoassay results. Here we define the general biochemical properties of antibodies and their similarities and differences, explain how these properties influence their functional relationship to an antigen target, and describe a method for the enzymatic fragmentation of antibodies into smaller functional parts. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 39 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 8 | 21% |
Student > Bachelor | 5 | 13% |
Student > Master | 4 | 10% |
Researcher | 3 | 8% |
Student > Doctoral Student | 2 | 5% |
Other | 3 | 8% |
Unknown | 14 | 36% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 8 | 21% |
Medicine and Dentistry | 4 | 10% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 8% |
Agricultural and Biological Sciences | 2 | 5% |
Immunology and Microbiology | 2 | 5% |
Other | 5 | 13% |
Unknown | 15 | 38% |