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Mendeley readers
| Chapter title |
Bioconjugation of Antibodies to Horseradish Peroxidase (HRP)
|
|---|---|
| Chapter number | 4 |
| Book title |
ELISA
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2742-5_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2741-8, 978-1-4939-2742-5
|
| Authors |
Robert M. Hnasko, Hnasko, Robert M. |
| Abstract |
The bioconjugation of an antibody to an enzymatic reporter such as horseradish peroxidase (HRP) affords an effective mechanism by which immunoassay detection of a target antigen can be achieved. The use of heterobifunctional cross-linkers to covalently link antibodies to HRP provides a simple and convenient means to maintain antibody affinity while imparting a functional reporter used for antigen detection. In this chapter, we describe a process by which Sulfo-SMCC is used to generate a stable maleimide-activated HRP that is reactive with sulfhydryl groups generated in antibodies by SATA-mediated thiolation. |
Mendeley readers
The data shown below were compiled from readership statistics for 49 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Austria | 1 | 2% |
| Unknown | 48 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 18% |
| Student > Bachelor | 7 | 14% |
| Student > Master | 7 | 14% |
| Researcher | 3 | 6% |
| Student > Doctoral Student | 2 | 4% |
| Other | 5 | 10% |
| Unknown | 16 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 18% |
| Agricultural and Biological Sciences | 6 | 12% |
| Immunology and Microbiology | 4 | 8% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 6% |
| Medicine and Dentistry | 3 | 6% |
| Other | 6 | 12% |
| Unknown | 18 | 37% |