| Chapter title |
Multiplexed Microsphere Suspension Array-Based Immunoassays
|
|---|---|
| Chapter number | 11 |
| Book title |
ELISA
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2742-5_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2741-8, 978-1-4939-2742-5
|
| Authors |
Andrew Lin, Alexandra Salvador, J. Mark Carter, Lin, Andrew, Salvador, Alexandra, Carter, J. Mark |
| Abstract |
ELISA is an extremely powerful tool to detect analytes because of its sensitivity, selectivity, reproducibility and ease of use. Here we describe sandwich immunoassays performed in suspension on spectrally unique microspheres developed by Luminex. Luminex assays offer the benefit of multiplex analysis of large numbers of analytes in a single reaction. Because the microspheres are spectrally unique, many microspheres, each attached to various antibodies, can be added to a single sample. Luminex instruments can distinguish each microsphere and detect the intensity of a reporter signal for each microsphere. Results are reported in Median Fluorescent Intensities for each analyte. Luminex assays can be used to detect up to 500 analytes in a high-throughput format. Luminex refers to this technology as xMAP(®). Here we describe a routine protocol for a Luminex immunoassay. Other Luminex assays would have to be optimized for specific conditions according to their use. |
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|---|---|---|
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|---|---|---|
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| Other | 2 | 10% |
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| Researcher | 2 | 10% |
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