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Mendeley readers
Attention Score in Context
| Chapter title |
The Western Blot
|
|---|---|
| Chapter number | 9 |
| Book title |
ELISA
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2742-5_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2741-8, 978-1-4939-2742-5
|
| Authors |
Thomas S. Hnasko, Robert M. Hnasko, Hnasko, Thomas S., Hnasko, Robert M. |
| Abstract |
Western blotting is a technique that involves the separation of proteins by gel electrophoresis, their blotting or transfer to a membrane, and selective immunodetection of an immobilized antigen. This is an important and routine method for protein analysis that depends on the specificity of antibody-antigen interaction and is useful for the qualitative or semiquantitative identification of specific proteins and their molecular weight from a complex mixture. This chapter will outline the requisite steps including gel electrophoresis of a protein sample, transfer of protein from a gel to a membrane support, and immunodetection of a target antigen. |
Mendeley readers
The data shown below were compiled from readership statistics for 483 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | <1% |
| United States | 1 | <1% |
| Egypt | 1 | <1% |
| Unknown | 480 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 134 | 28% |
| Student > Master | 52 | 11% |
| Student > Ph. D. Student | 29 | 6% |
| Student > Doctoral Student | 12 | 2% |
| Student > Postgraduate | 12 | 2% |
| Other | 29 | 6% |
| Unknown | 215 | 45% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 108 | 22% |
| Agricultural and Biological Sciences | 36 | 7% |
| Medicine and Dentistry | 32 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 15 | 3% |
| Neuroscience | 12 | 2% |
| Other | 52 | 11% |
| Unknown | 228 | 47% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 September 2022.
All research outputs
#20,777,031
of 23,381,576 outputs
Outputs from Methods in molecular biology
#10,122
of 13,334 outputs
Outputs of similar age
#299,158
of 356,103 outputs
Outputs of similar age from Methods in molecular biology
#651
of 1,005 outputs
Altmetric has tracked 23,381,576 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,334 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 356,103 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,005 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.