Report for: ELISA

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Chapter title	Direct ELISA
Chapter number	6
Book title	ELISA
Published in	Methods in molecular biology, January 2015
DOI	10.1007/978-1-4939-2742-5_6
Pubmed ID	26160564
Book ISBNs	978-1-4939-2741-8, 978-1-4939-2742-5
Authors	Alice V. Lin, Lin, Alice V.
Abstract	First described by Engvall and Perlmann, the enzyme-linked immunosorbent assay (ELISA) is a rapid and sensitive method for detection and quantitation of an antigen using an enzyme-labeled antibody. Besides routine laboratory usage, ELISA has been utilized in medical field and food industry as diagnostic and quality control tools. Traditionally performed in 96-well or 384-well polystyrene plates, the technology has expanded to other platforms with increase in automation. Depending on the antigen epitope and availability of specific antibody, there are variations in ELISA setup. The four basic formats are direct, indirect, sandwich, and competitive ELISAs. Direct ELISA is the simplest format requiring an antigen and an enzyme-conjugated antibody specific to the antigen. This chapter describes the individual steps for detection of a plate-bound antigen using a horseradish peroxidase (HRP)-conjugated antibody and luminol-based enhanced chemiluminescence (ECL) substrate. The methodological approach to optimize the assay by chessboard titration is also provided.

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Mendeley readers

The data shown below were compiled from readership statistics for 246 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country	Count	As %
Unknown	246	100%

Demographic breakdown

Readers by professional status	Count	As %
Student > Bachelor	55	22%
Student > Master	28	11%
Student > Doctoral Student	9	4%
Unspecified	9	4%
Researcher	9	4%
Other	24	10%
Unknown	112	46%

Readers by discipline	Count	As %
Biochemistry, Genetics and Molecular Biology	50	20%
Agricultural and Biological Sciences	20	8%
Unspecified	10	4%
Chemistry	9	4%
Pharmacology, Toxicology and Pharmaceutical Science	8	3%
Other	34	14%
Unknown	115	47%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 March 2016.

All research outputs

#7,554,098

of 23,043,346 outputs

Outputs from Methods in molecular biology

#2,342

of 13,194 outputs

Outputs of similar age

#106,471

of 354,277 outputs

Outputs of similar age from Methods in molecular biology

#169

of 998 outputs

Altmetric has tracked 23,043,346 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.

So far Altmetric has tracked 13,194 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done well, scoring higher than 76% of its peers.

Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 354,277 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 54% of its contemporaries.

We're also able to compare this research output to 998 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 77% of its contemporaries.

ELISA

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Attention Score in Context